Skip to main content
SpringerLink
Log in

Ionic liquid/graphene oxide as a nanocomposite for improving the direct electrochemistry and electrocatalytic activity of glucose oxidase

  • Original Paper
  • Published:
Journal of Solid State Electrochemistry Aims and scope Submit manuscript

Abstract

By combination of 1-ethyl-3-methyl immidazolium ethyl sulfate as a typical room temperature ionic liquid (IL) and graphene oxide (GO) nanosheets, a nanocomposite was introduced for improving the direct electrochemistry and electrocatalytic activity of glucose oxidase (GOx). The enzyme on the IL–GO-modified glassy carbon electrode exhibited a quasireversible cyclic voltammogram corresponding to the flavine adenine dinucleotide/FADH2 redox prosthetic group of GOx. At the scan rate of 100 mV s−1, the enzyme showed a peak-to-peak potential separation of 82 mV and the formal potential of −463 mV (vs Ag/AgCl in 0.1 M phosphate buffer solution, pH 7.0). The kinetic parameters of the charge transfer rate constant, the electron transfer coefficient, and the apparent Michaelis–Menten constant were calculated as 1.36 s−1 and 0.35 and 2.47 μM, respectively. When the modified electrode was examined as a biosensor for glucose determination, a linear range of 2.5–45 nM with detection limit of 0.175 nM (signal to noise = 3) was obtained. The biosensor was stable for 2 months.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Lee SR, Lee YT, Sawada K, Takao H, Ishida M (2008) Biosens Bioelectron 24:410–414

    Article  CAS  Google Scholar 

  2. Newman JD, Turner APF (2005) Biosens Bioelectron 20:2435–2453

    Article  CAS  Google Scholar 

  3. Wu L, Zhang X, Ju H (2007) Biosens Bioelectron 19:141–147

    Google Scholar 

  4. Du P, Zhou B, Cai CX (2008) J Electroanal Chem 614:149–156

    Article  CAS  Google Scholar 

  5. Jia WZ, Hu YK, Song YY, Wang K, Xia XH (2008) Biosens Bioelectron 23:892–898

    Article  CAS  Google Scholar 

  6. Kohma T, Oyamatsu D, Kuwabata S (2007) Electrochem Commun 9:1012–1016

    Article  CAS  Google Scholar 

  7. Lin YH, Lu F, Tu Y, Ren ZF (2004) Nano Lett 4:191–195

    Article  CAS  Google Scholar 

  8. Wang J (2008) Chem Rev 108:814–825

    Article  CAS  Google Scholar 

  9. Kang X, Wang J, Wu H, Aksay IA, Liu J, Lin Y (2009) Biosens Bioelectron 25:901–905

    Article  CAS  Google Scholar 

  10. Zhang X, Wang J, Ogorevc B, Spichiger UE (1999) Electroanalysis 11:945–949

    Article  CAS  Google Scholar 

  11. Chang HX, Tang LH, Wang Y, Jiang JH, Li JH (2010) Anal Chem 82:2341–2346

    Article  CAS  Google Scholar 

  12. Li YM, Tang LH, Li JH (2009) Electrochem Commun 11:846–849

    Article  Google Scholar 

  13. Novoselov KS, Geim AK, Morozov SV, Jiang D, Zhang Y, Dubonos SV, Grigorieva IV, Firsov AA (2004) Science 306:666–669

    Article  CAS  Google Scholar 

  14. Wu ZS, Pei SF, Ren WC, Tang DM, Gao LB, Liu BL, Li F, Liu C, Cheng HM (2009) Adv Mater 21:1–5

    CAS  Google Scholar 

  15. Liua S, Tiana J, Wanga L, Luoa Y, Lua W, Sun X (2011) Biosens Bioelectron 26:4491–4496

    Article  Google Scholar 

  16. Cai W, Piner RD, Stadermann FJ, Park S, Shaibat MA, Ishii Y, Yang D, Velamakanni A, An SJ, Stoller M, An J, Chen D, Ruoff RS (2008) Science 321:1815–1817

    Article  CAS  Google Scholar 

  17. He H, Klinowski J, Forster M, Lerf A (1998) Chem Phys Lett 287:53–56

    Article  CAS  Google Scholar 

  18. Zuo X, He S, Li D, Peng C, Huang Q, Song S, Fan C (2010) Langmuir 26:1936–1939

    Article  CAS  Google Scholar 

  19. Lu W, Luo Y, Chang G, Sun X (2011) Biosens Bioelectron 26:4791–4797

    Article  CAS  Google Scholar 

  20. Zhao F, Wu XE, Wang MK, Liu Y, Gao LX, Dong SJ (2004) Anal Chem 76:4960–4967

    Article  CAS  Google Scholar 

  21. Kachoosangi RT, Musameh MM, Yousef IA, Yousef JM, Kanan SM, Xiao L, Davies SG, Russell A, Compton RG (2009) Anal Chem 81:435–442

    Article  CAS  Google Scholar 

  22. Zhao GC, Xu MQ, Ma J, Wei XW (2007) Electrochem Commun 9:920–924

    Article  CAS  Google Scholar 

  23. Shangguan X, Zhang H, Zheng J (2008) Electrochem Commun 10:1140–1143

    Article  CAS  Google Scholar 

  24. Dai Z, Xiao Y, Yu X, Mai Z, Zhao X, Zou X (2009) Biosens Bioelectron 24:1629–1634

    Article  CAS  Google Scholar 

  25. Shiddiky JAM, Torriero AJA (2011) Biosens Bioelectron 26:1775–1787

    Article  CAS  Google Scholar 

  26. Li J, Yu J, Zhao F, Zeng B (2007) Anal Chim Acta 587:33–40

    Article  CAS  Google Scholar 

  27. Rahimi P, Rafiee-Pour H, Ghourchian H, Norouzi P, Ganjali MR (2010) Biosens Bioelectron 25:1301–1306

    Article  CAS  Google Scholar 

  28. Zhang Y, Shen Y, Han D, Wang Z, Song J, Li F, Niu L (2007) Biosens Bioelectron 23:438–443

    Article  Google Scholar 

  29. Wu X, Zhao B, Wu P, Zhang H, Cai C (2009) J Phys Chem B 113:13365–13373

    Article  CAS  Google Scholar 

  30. Zhilei W, Zaijun L, Xiulan S, Yinjun F, Junkang L (2010) Biosens Bioelectron 25:1434–1438

    Article  Google Scholar 

  31. Liu Y, Liu L, Dong S (2007) Electroanalysis 19:55–59

    Article  CAS  Google Scholar 

  32. Choi BG, Park HS, Park TJ, Kim DH, Lee SY, Hong WH (2009) Electrochem Commun 11:672–675

    Article  CAS  Google Scholar 

  33. Liu Y, Zou X, Dong S (2006) Electrochem Commun 8:1429–1433

    Article  CAS  Google Scholar 

  34. Hummers WS, Offeman RE (1958) J Am Chem Soc 80:1339–1339

    Article  CAS  Google Scholar 

  35. Wang J, Musameh M (2005) Anal Chim Acta 539:209–13

    Article  CAS  Google Scholar 

  36. Manso J, Mena ML, Yáñez-Sedeño P, Pingarrón J (2007) J Electroanal Chem 603:1–7

    Article  CAS  Google Scholar 

  37. Qiu JD, Zhou WM, Guo J, Wang R, Liang RP (2009) Anal Biochem 385:264–9

    Article  CAS  Google Scholar 

  38. Chu X, Duan D, Shen G, Yu R (2007) Talanta 71:2040–7

    Article  CAS  Google Scholar 

  39. Cai CX, Chen J (2004) Anal Biochem 332:75–83

    Article  CAS  Google Scholar 

  40. Guiseppi-Elie A, Lei CH, Baughman RH (2002) Nanotechnology 13:559–564

    Article  CAS  Google Scholar 

  41. Liu Y, Wang MK, Zhao F, Xu ZA, Dong SJ (2005) Biosens Bioelectron 21:984–988

    Article  CAS  Google Scholar 

  42. Baldwin RL (1996) Biophys J 71:2056–2063

    Article  CAS  Google Scholar 

  43. Bauduin P, Renoncourt A, Touraud D, Kunz W, Ninham BW (2004) Curr Opin Colloid Interface Sci 9:43–47

    Article  CAS  Google Scholar 

  44. Collins KD (2004) Methods 34:300–311

    Article  CAS  Google Scholar 

  45. Von Hippel PH, Wong KY (1965) J Biol Chem 240:3909–3923

    Google Scholar 

  46. Wiggins PM (1997) Physica A 238:113–128

    Article  CAS  Google Scholar 

  47. Rahimi P, Ghourchian H, Sajjadi S (2012) Analyst 137:471–5

    Article  CAS  Google Scholar 

  48. Bond AM (1980) Modern polarographic methods in analytical chemistry. Dekkes, New York

    Google Scholar 

  49. Laviron E (1979) J Electroanal Chem 101:19–28

    Article  CAS  Google Scholar 

  50. Pang X, He D, Luo S, Cai Q (2009) Sensors Actuators B 137:134–8

    Article  Google Scholar 

  51. Tsai MC, Tsai YC (2009) Sensors Actuators B 141:592–8

    Article  Google Scholar 

  52. Gao Y, Shen C, Di J, Tu Y (2009) Mater Sci Eng C 29:2213–6

    Article  CAS  Google Scholar 

  53. Wu XE, Zhao F, Varcoe JR, Thumser AE, Avignone-Rossa C, Slade RCT (2009) Bioelectrochemistry 77:64–8

    Article  CAS  Google Scholar 

  54. Shu FR, Wilson GS (1976) Anal Chem 48:1679–1686

    Article  CAS  Google Scholar 

  55. Wang B, Li B, Deng Q, Dong S (1998) Anal Chem 70:3170–3174

    Article  CAS  Google Scholar 

  56. Vidal J, Garcia E, Castillo JR (1998) Biosens Bioelectron 13:371–382

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial support of the Research Councils of Sharif University of Technology and University of Tehran are gratefully acknowledged.

Author information

Authors and Affiliations

  1. Department of Chemistry, Shahid Beheshti University, Tehran, Iran

    Mahboubeh Tasviri

  2. Department of Chemistry, Sharif University of Technology, Azadi Ave., Tehran, Islamic Republic of Iran

    Shahnaz Ghasemi & Mohammad Reza Gholami

  3. Institute of Biochemistry & Biophysics, University of Tehran, Enghelab Ave., Tehran, Islamic Republic of Iran

    Hedayatollah Ghourchian

Authors
  1. Mahboubeh Tasviri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shahnaz Ghasemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hedayatollah Ghourchian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Reza Gholami
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Mahboubeh Tasviri, Hedayatollah Ghourchian or Mohammad Reza Gholami.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

DOC 343 kb

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tasviri, M., Ghasemi, S., Ghourchian, H. et al. Ionic liquid/graphene oxide as a nanocomposite for improving the direct electrochemistry and electrocatalytic activity of glucose oxidase. J Solid State Electrochem 17, 183–189 (2013). https://doi.org/10.1007/s10008-012-1858-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10008-012-1858-5

Keywords

Search

Navigation